Cloning and expression analysis of vasa during the reproductive cycle of Korean rockfish, Sebastes schlegeli     

MU Weijie  WEN Haishen  HE Feng  LI Jifang  LIU Miao  MA Ruiqin  ZHANG Yuanqing  HU Jian  QI Baoxia 《中国海洋大学学报(英文版)》2013,12(1):115-124

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Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus     

ZANG Xiaonan  ZHANG Xuecheng  MU Xiaosheng  LIU Bin 《中国海洋大学学报(英文版)》2013,12(1):169-174

This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mgL-1) than that from soluble protein (1.964 mgL-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture.  相似文献   

基准面旋回对河流相储层物性差异及声波测井影响     

梁宏伟  吴胜和  穆龙新  岳大力  李宇鹏  印森林  喻宸 《地球科学》2013,38(5):1135-1142

为揭示基准面旋回对不同旋回阶段河流相储层物性差异及声波时差测井响应的影响,运用基准面旋回沉积动力学原理,对岩心分析化验数据等实测资料进行分析,对孤岛油田中一区馆陶组上段3砂层组河流相储层物性差异主控因素进行分析,明确了基准面旋回对河道微相在不同旋回阶段储层物性差异和声波测井响应的控制机理,并确定了不同级次基准面旋回内河道储层物性分布特征和声波测井响应差异.结果表明:不同级次基准面升降引起可容纳空间与物源供给条件的改变,导致不同旋回阶段河道储层沉积环境出现差异,使短期基准面旋回内部单河道内部储层物性正韵律分布、声波测井响应差异不大;中期基准面旋回中组成复合河道的多期单河道间储层物性自下向上呈总体变好趋势、声波测井响应有异;长期基准面旋回中多期复合河道间储层物性呈无规则分布、声波测井响应差异明显.   相似文献   

三疣梭子蟹蜕壳后肝胰腺消化酶活力变化特征研究     

林鹏  刘华  余作奔  母昌考  宋微微  李荣华  王春琳 《海洋学研究》2013,31(4):74-79

消化酶是反映机体消化生理状况及对营养物质利用能力的重要指标。本文对三疣梭子蟹蜕壳后不同时间肝胰腺消化酶活力进行了研究,结果显示,蜕壳后肝胰腺中胃蛋白酶、胰蛋白酶和淀粉酶活力在第4~6天达到最高;脂肪酶活力在第6天时达到最高;纤维素酶活力总体较低,在第10~12天达到最高;几丁质酶活力在蜕壳后第4天时酶活力最高。研究表明,三疣梭子蟹蜕壳后,胰蛋白酶和胃蛋白酶活力相对较高,而纤维素酶和淀粉酶活力较低,可能与三疣梭子蟹本身的肉食性特征有关;另外,整个实验期间,三疣梭子蟹肝胰腺脂肪酶活力较低,可能与测定条件有一定的关系。与其他时间点相比,蜕壳后4~6 d(蜕壳周期中间阶段)各种酶活力处于较高水平,表明此阶段对食物的消化能力比其他时间更强,这为养殖过程中饵料的合理投喂提供了参考依据。  相似文献   

一次沙尘过程对天津气溶胶浓度分布的影响   总被引：1，自引：1，他引：0  

姚青  蔡子颖  韩素芹  穆怀斌 《中国沙漠》2013,33(4):1138-1143

利用气溶胶质量浓度和数浓度监测资料以及不同高度的常规气象资料,结合后向轨迹模式,分析2011年4月30日至5月1日一次沙尘天气过程对天津城区气溶胶浓度的影响。结果表明：沙尘过程前的轻雾天气下PM1贡献了气溶胶质量浓度的96%和数浓度的99.9%以上;本次沙尘天气存在两个不同的浮尘过程,主要区别体现在细粒子浓度差异上,第一次浮尘过程PM1~2.5、PM2.5~10和PM10~100分别占气溶胶数浓度的6.5%、2.5%和0.1%,第二次浮尘过程占比则依次为11.3%、2.6%和0.01%;两次浮尘过程气溶胶粒子性质有明显差异,第一次浮尘过程中粗粒子浓度占PM10的80%以上,第二次浮尘过程风向转变为偏北风,细粒子浓度增高至40%,气溶胶由单纯的沙尘气溶胶转变为沙尘-污染气溶胶。  相似文献   

西北地区东部季节干旱的时空变化特征分析     

杨建玲  冯建民  穆建华  郑广芬  谭志强  王素艳 《冰川冻土》2013,35(4):949-958

干旱作为我国西北地区东部影响最大的气象灾害, 可引起农业减产、水资源短缺、土地荒漠化和生态环境恶化等严重问题.在国家新一轮西部大开发战略实施之初, 在全球气候变暖背景下, 有必要对干旱发展的最新特征和演变趋势进行详细分析研究, 为加强防旱、抗旱,促进经济发展提供科学决策依据和参考.采用国家干旱标准综合干旱指数(CI指数), 利用西北地区东部74个气象代表站逐日气温、降水资料, 分析了西北地区东部不同级别干旱日数在各个季节的时空分布和变化趋势. 结果表明: 在气候变暖背景下, 西北地区东部从长期趋势看, 春、夏、秋季干旱呈加剧趋势, 冬季干旱呈减轻趋势. 21世纪以来春、夏季干旱进一步加剧, 尤其是夏季加剧更显著, 而秋、冬季干旱出现了减弱的新趋势. 在西北地区东部主降水期3-11月重-特旱加剧趋势比轻-中旱加剧显著, 南部干旱化趋势比北部更加明显. 尤其是宁夏同心地区春旱加剧非常显著, 已成为西北地区东部重-特旱最严重的地区.对于干旱发展的这一新动态, 必须引起有关部门的高度重视, 采取科学、有效手段加强防旱、抗旱.  相似文献   

塔克拉玛干沙漠腹地大气气溶胶中游离氧化铁的测定     

陆辉  魏文寿  刘明哲  吴新萍  牟书勇  韩茜 《地理科学》2011,31(8):969-975

大气沙尘气溶胶中铁氧化物含量的测定对研究气溶胶吸收特性有重要的研究意义。采用柠檬酸盐-重碳酸盐-连二亚硫酸盐(CBD)和漫反射光谱法(DRS)对塔克拉玛干沙漠腹地塔中站2006年4月9~11日沙尘暴天气过程前后TSP沙尘样品中的游离氧化铁进行定量和半定量研究,研究得出,塔中游离氧化铁占全铁含量的变化范围在4.55%~8.16%之间(平均值6.36%),全铁占样品总量的5.16%,游离氧化铁占样品总量的0.32%;反射光谱一阶导数曲线峰值在435和560nm对针铁矿和赤铁矿具有识别指示意义。  相似文献   

The 2008 Kultuk earthquake with M w = 6.3 in the south of Baikal: Spatial-temporal analysis of seismic activation     

V. I. Mel’nikova  N. A. Gileva  S. S. Aref’ev  V. V. Bykova  O. K. Masal’skii 《Izvestiya Physics of the Solid Earth》2012,48(7-8):594-614

The results of investigating the data of stationary and field observations in the epicentral zone of the Kultuk earthquake of August 27, 2008 with M _w = 6.3 localized south of Baikal are presented. The seismic activation amounting to 1790 aftershocks with K _p ?? 4 (M _w ?? 0.9) affected a part of the general fault bounding the southwestern coast of the lake and shallower intrabasin structures. It was established through the cluster analysis that the main shock was located at the periphery of the cloud of the concentrated seismicity component, and three main clusters reflecting a complex character of rupture in the earthquake source were identified in the zone of aftershocks. Owing to a high accuracy of aftershock hypocenters determinations (ERZ ?? 1.2 km), the local character of the seismisity-generating zones was revealed. Based on the method of mapping seismic regime parameters, it was established that zones of reduced fractionality are recognizable in the central part of the source area; in this case, the entire periphery, except for the southeastern direction (where the main shock epicenter is located), is characterized by a high fractionality, which also points to the complex character of the rupture in the source. On the whole, the aftershock sequence under consideration showed the activation of the southwestern flank of the Southern Baikal region, which remained passive for more than one century, and demonstrated destructive features of the Earth??s crust.  相似文献   

煤矿矿震定位中异向波速模型的构建与求解   总被引：5，自引：2，他引：3       下载免费PDF全文

巩思园  窦林名  马小平  牟宗龙  贺虎  何江 《地球物理学报》2012,55(5):1757-1763

针对煤矿上覆岩层层状赋存和离层带的特点,构建矿井尺度的微震监测系统异向波速模型,模型中波速向量由地面探头速度与井下探头速度组成.研究了在只有强矿震信号和混有爆破信号两种条件下,以到时残差最小为目标和震源定位误差最小为目标的两种求解模型,模型求解选用具有全局寻优特性的遗传算法与CMEAS算法结合的混合算法.现场实际应用得出,只使用爆破信号的到时残差法最优,混有强矿震信号的到时残差法其次;与爆破信号定位所用的统一简化波速模型相比,震源定位误差大幅度降低.在此基础上进一步减低定位误差,还需从微震台网的优化布设方面解决.  相似文献   

Concentration of selected radionuclides in seawater from Kuwait     

Uddin S  Al Ghadban AN  Aba A  Behbehani M 《Marine pollution bulletin》2012,64(6):1261-1264

No baseline existed for the radionuclides in Kuwait territorial water. With changing trend in the region to embrace nuclear energy, the baseline study is imperative to create a reference and to record the influence-functioning of upcoming power plants. The first one in Bushehr, Iran is ready to start and several more are likely to come-up in UAE, Saudi Arabia and Kuwait. The present baseline concentration of the four considered radionuclide's show low concentration of tritium, polonium, strontium and cesium; their concentration is comparable to most oceanic waters.  相似文献